Gel permeation chromatography (GPC)
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Gel permeation chromatography (GPC)

Chromatography involves separating out a mixture, typically by passing it through a separation medium that gradually traps solutes due to their size – in Gel permeation chromatography (GPC) a sample is dissolved in suitable solvent and passed through a gel-based separation medium.This could be done immediately ahead of analysis step, perhaps to see how much of a specific protein is within a sample, or it could be done to synthesise a highly purified material for later fabrication steps.

List of available equipment
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Agilent Technologies 5975c/7890a
Gel permeation chromatography (GPC)
Materials Node University of Wollongong
Description
GCMS - HPLC for Gel permeation chromatography
Related Information
Optimised for Gel Permeation Chromatography - polymer molecular weight determination.
Tool Contact
sbeirne@uow.edu.au
Waters 1515 Isocratic HPLC Pump
Gel permeation chromatography (GPC) for organic materials
QLD Node University of Queensland
Description
Separation and analysis of molecular weight and PDI of organic compounds or polymers that have been dissolved in Tetrahydrofuran (THF).
Related Information
Used to analyse biopolymers and during the synthesis of organic compounds. Maximum molecular weight range of 500 to 1.5M Daltons (detector dependant).
Tool Contact
anff@uq.edu.au
Agilent Technologies 1260 Infinity (GPC)
Aqueous gel permeation chromatography (GPC) system with RI, UV, viscosity and light scattering detectors
QLD Node University of Queensland
Description
Four detector system to separate and analyse molecular weight and PDI of water soluble compounds or polymers.
Related Information
Commonly used to study biomaterials with a maximum molecular weight range of 500 to 1.2M Daltons.
Tool Contact
anff@uq.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Agilent Technologies 5975c/7890a
Gel permeation chromatography (GPC)
QLD Node University of Queensland
Description
GCMS - HPLC for Gel permeation chromatography
Related Information
Optimised for Gel Permeation Chromatography - polymer molecular weight determination.
Tool Contact
sbeirne@uow.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Waters 1515 Isocratic HPLC Pump
Gel permeation chromatography (GPC) for organic materials
QLD Node University of Queensland
Description
Separation and analysis of molecular weight and PDI of organic compounds or polymers that have been dissolved in Tetrahydrofuran (THF).
Related Information
Used to analyse biopolymers and during the synthesis of organic compounds. Maximum molecular weight range of 500 to 1.5M Daltons (detector dependant).
Tool Contact
anff@uq.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Agilent Technologies 1260 Infinity (GPC)
Aqueous gel permeation chromatography (GPC) system with RI, UV, viscosity and light scattering detectors
QLD Node University of Queensland
Description
Four detector system to separate and analyse molecular weight and PDI of water soluble compounds or polymers.
Related Information
Commonly used to study biomaterials with a maximum molecular weight range of 500 to 1.2M Daltons.
Tool Contact
anff@uq.edu.au