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Microfluidic experimentation station 1

1. Nikon inverted microscope with phase contrast and fluorescence equipped with phase shift objectives which includes 4X, 10X, 20X, 40X, 60X and 100X objectives with long working distance that are essential for microfluidic experiments. There is an anti-vibration optical table to eliminate any shocks during experiments. This microscope is equipped with 200 Hour fiber optic light source and monochrome cooled digital camera with standard research software for analysis of images.

2. A Phantom V9 high speed color camera from Vision research, with speeds of 1000 frames per second at the full resolution of 1632 x 1200 pixels and of 150,000 frame per second at lowest resolution of 96 X 8 pixels. The Phantom V9.1 is the only 2-mega-pixel camera where the vertical resolution is greater than 1080 pixels (1200 pixels). This camera is most popular in the field of micro-fluidics due to its high vertical resolution. The Phantom V9.1 camera has a high internal memory, that is useful when creating large movies (30 to 60 sec) required for most of the microfluidic applications.

3. Microfluidic controller, MFCS-FLEX-8C-1000 mbar system, from Fluigent, France, configured with 8 independent channels with positive pressure from 0 up to 1000 mbar at 20psi of compressed air pressure, two Fluiwell-4C-2 ml with 4 vials of 2 ml each, to pressurize microfluidics device. The system is also equipped with Flowell equipment configured with 3 channels to characterize the flow rate in microfluidic channel from 2 nl/mn up to 7 µl/mn. The Maesflo software allows controlling and fully regulating flow rate on three channels of the Fluiwell and the MFCS with precise feed-back on pressure. Fluigent MFCS allow pulse less fluid dispense to microfluidic device when compared to syringe pumps. These systems have high stability (<0.1%) and instantaneous response time (<200 ms) with high reliability and quality. This microfluidic experiment station will be available for our ever growing academic client base in the area of microfluidics, especially pressure driven microfluidic devices.

4. Microfluidic valve controller (24 channels) system is build on the platform supplied by Dr. Rafael Gómez-Sjöberg, Quake Lab, Stanford University. All 24 channels can be controlled independently for certain microfluidic application where microvalve are used for plumbing of microchannels. This particular piece of in-house equipment cannot provide varying pressure similar to fluigent MFCS system.

Fabrication step: Device fabrication, packaging and testing
Function: Microfluidics
Location: University of Queensland
Node: Queensland