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Confocal Microscope

With the use of Laser Scanning Confocal Microscopy (LSCM), the ability to view fluorescent specimens for biological and biomedical applications has largely increased over conventional optical microscopy. LSCM incorporates solid-state lasers to irradiate and excite specimen at specific wavelengths, with the ability to view samples at an axial and lateral resolution of 200nm.

The Nikon A1Rsi Confocal Microscope is attached to an inverted microscope, where a laser is combined with photomultiplier tube detector and images are collected and displayed on a computer. Various software can be used to process the data.

Fabrication step: Characterisation
Function: Microscopy
Location: Melbourne Centre for Nanofabrication
Purpose: The LSCM has the capabilities to scan single optical sections as well as a sequence of sections to view different levels and thus attain a three-dimensional image. With the ability to image fixed and living cells, a time-lapse study with improved resolutions is also easily achieved. The LSCM at MCN can also be integrated with the JPK Atomic Force Microscope.
Material systems: Biological and all other materials
Node: Victoria